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1. Assume you are looking for a particular pathogen in a sample and you are using a highly selective medium, and so you make five 1:10 dilutions of the sample and you plate 0.2 mLs of each dilution. The following day you find that you have 80, 50, 25, and 1 colonies on plates 2, 3, 4, and 5. How many pathogens per mL do you have?
2. You have 1mL containing 10^8 cells and you need 2.5x10^7 cells. What do you do? (how many cells in each 0.1mL)
3. You have 10 mL of 10^6 cells/mL prep and you need 1mL of 10^7 cells. What do you do? Hint: you can se cetrifugation, but then you have to perform another step)
4. You are generating a vaccine prep that contains 10^8 microbes per mL and you need to know the ID50 of your prep. So you run a challenge study with 50 mice where you inoculate each mouse with 50 uL of the prep. Within 3 days you record the number of sick mice and you find that 30 of the mice are sick. How can you estimate the ID50, and what is that estimate? (use a ration)
What techniques would you utilize to detect this DNA sequence. Mechanical stress or trauma frequently triggers the symptoms of gouty arthritis in susceptible people. In this view elucidate why gouty arthritis affects the great toe more commonly than ..
Compare and contrast, in depth, the cellular processes of mitosis and meiosis. Include interphase in your explanation. Chromosomal movement and status must be included in your answer. You may include a diagram to explain your answer but you should..
Find out the number of triplet codons feasible
Modern theory suggests that the early pre-life atmosphere on Earth was a reducing one. Why is it believed that oxygen was not present as life formed on Earth.
Suppose a population evolving by genetic drift in which the frequency of allele K is 0.65. What is the probability that at some point in the future allele K will drift to a frequency of 1?
define the difference between a tumor suppressor and an oncogene with respect to the actual causes of cancer?
Find out both the allelic frequencies and the genotypic frequencies. A 68-year-old client was admitted to the hospital medical floor with a diagnosis of chronic bronchitis. His wife asks the nurse why his skin looks blue. How would you describe cyano..
Why is DNA able to store large amounts of knowledge
Most of the genome does not code for protein, therefore 99% of the human genome is junk that serves no purpose?. With specific reference to examples long and short non-coding RNAs discuss whether you agree with this statement. In your answer you shou..
There are several examples of eukaryotic cells. Algae, Fungi, Plants, and Animals are all comprises of eukaryotic cells.Select either a plant or an animal cell.
Phenylalanine residues are often found in what general locations in water soluble globular proteins?
The Little Bacterium That Cannot. Acholeplasma laidlawii is small bacterium that cannot synthesize its own fatty acids and must therefore construct its plasma membrane
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