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A biologist was testing E. coli for thymidine requirement. She found a thy- point mutant and wanted to study reversion to thy+. She grew 20 cultures of the thy- mutant strain and plated them onto 20 plates that contained media to select for thy+ revertants. She obtained a total of 460 colonies. Six of the plates showed no colonies.a) If 5 x 108 cells were spread on each plate what was the reversion frequency and the reversion rate?b) Explain what steps she could have taken to find the thy- mutant in the first place, and why she did each step.c) She also wanted to study the type of mutation that occurred during formation of the original thy- cells. The mutagens HA (a base modifyer), BU (a base analogue), and acridine (an intercalator) were each added to thy- cells. Only BU reverted the thy- mutants to WT. Briefly describe the types of mutations each mutagen causes. Based on this information what was the original mutation to thy- to begin with? Explain your reasoning.
Discuss how would one test the hypothesis about maximizing efficiency of food finding and delivery when discussing species like the boobies or egrets
When did dinosaurs and pterosaurs become extinct? Which are the only extant animals that descended directly from dinosaurs.
Explain the arrangement within energy levels of the six electrons of an atom of carbon and discuss the relationship between elements and atoms?
Describe why organisms only reproduce their own kind, and why offspring more closely resemble their parents than unrelated individuals of the similar species.
Amino acid compositions can be determined by heating a protein in 6 M HCL and running the hydrolysate through an ion-exhange column. If you were going to do an amino acid sequencing experiment, why would you want to get an amino acid composition f..
A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also contains 300mM imidazole.
A new enzyme you are studying has a Km of 7.0mM and a kcat of 126 per second. What will be the initial rate of reaction in units mM/min when 9.3 nanometer enzyme is reacted with 2.7 mM substrate. Report your answer in units if mM/min to nearest 1m..
If the fox were to feed only on insect-eating birds, how many square meters of plant material would be necessary.
White tigers are indicated with unshaded symbols. (As you can see, there was considerable inbreeding in this lineage. For instance, the white tiger Mohan was mated with his daughter). In answering the questions, assume that hite.
What phenotypes would you expect in the F2, and in what actual numbers (not ratios) would you be expecting to find them. Describe the process of speciation. Comprise in the discussion the factors that can contribute to the maintenance of genetic isol..
Name three bodily fluids, besides blood serum, that might be used for immune testing. How do you know a reaction between specific antibody and antigen has occurred in an ELISA test
Aphids can reproduce sexually and asexually. Females reproduce by themselves and give birth to live females in summer. They also reproduce sexually and lay eggs that survive in summer and eggs that survive in the winter and hatch in the spring.
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