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Q. You are working in a human genetics laboratory that studies causes and treatments for eye cataracts in newborns. This disease is thought to be caused by a deficiency in an enzyme called galactokinase, but the human gene that encodes this enzyme has not yet been identified. At a talk by a visiting scientist, you learn about a strain of bakers' yeast that contains a mutation called gal1- in its galactokinase gene. Because this gene is needed to metabolize galactose, the mutant strain cannot grow in galactose medium. Knowing that all living things evolved from a common ancestor and that distantly related organisms often have homologous genes that perform similar functions, you wonder if the human galactokinase gene can function in yeast. Because you have an optimistic temperament, you decide to pursue this line of experimentation. You isolate mRNA gene transcripts from human cells, use reverse transcriptase to make complementary DNA (cDNA) copies of the mRNA molecules, and ligate the cDNAs into circular plasmid DNA molecules that can be stably propagated in yeast cells. You then transform the pool of plasmids into gal1- (gal1 minus) yeast cells so that each cell receives a single plasmid. What do you think will happen when you spread the plasmidcontaining cells on Petri plates that contain galactose as a carbon source? How could this approach help you find the human gene encoding galactokinase?
In a population you count 30 red plants, 50 pink plants, and 20 white plants. What are the allele frequencies of the CR and CW alleles in this population.
Action potentials are usually generated at axon hillock and propagated down axon away from the cell body. If you experimentally depolarized an axon to the threshold level at a point halfway down the axon, what would happen.
What defence was successful in preventing you from grab the snake. While travelling in Texas, you stumble across a snake with red, yellow, and black bands.
What is an advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein.
Explain this dosage strategy in terms of the negative feedback control of cortisol secretion and receptor function in hormonal pathways.
The top of mountain is at an elevation of 45° from one bank of a river and at an elevation of 60° from the other bank. If river has a width of 1 km, what is the height [in km] of the mountain.
How do you describe the apparent contradictory finding of spontaneous pain and interference with pain perception in just about the same area.
The group of molecular biologists is trying to synthesize a new artificial compound to mimic the effects of a known hormone that influences sexual behaviour. They have turned to you for advice.
Male frogs give calls that attract female frogs to approach and mate. Researchers examined mating calls of closely related tree frogs in South America. What outcomes be able to occur where the ranges of two species overlap.
You cross an M1M2 father with an M2M3 mother and ?nd out that the mean number of nosehairs in dissimilar marker genotypes in their offspring are as follows.
Which condition is best described while the ovaries shut down their production of eggs and hormones like estrogens and progesterone approximately 30 years after they started.
Why can there be dissimilarity between heart rate averaged over 15 seconds and instantaneous heart rates. Suppose that green turtle grass ingestion time for the sea urchins has an approximate normal distribution.
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