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In the progress of performing an experiment, we find that (briefly) washing cells in a buffered solution at pH 3 is effective for isolating a cell membrane associated hormone receptor. (A std growth soln for the cells is usually pH 7).
1. Explain what must be true regarding the protein.
2. Explain the chemical changes that are occurring by the alteration of the pH to result in isolation of this protein.
Name three bodily fluids, besides blood serum, that might be used for immune testing. How do you know a reaction between specific antibody and antigen has occurred in an ELISA test
The product of the complete hydrolysis contained 2.2 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the ?-amino groups of other amino acids could be found. How many polypeptide chains are in the protein?
Explain center of gravity and base of support. How do these concepts relate to balance?
How do you determine the allelic frequency, expected genotype frequency if the population were all genetic equilibrium, and compare the calculated expected genotypic frequencies.
(Assume that one-half of the volume of the cell is composed of organelles, and thus not available for diffusion of glucose)
Isra fills two identical balloons at identical temperatures. She fills one with O2 gas and the other with O3 gas. Which balloon will deflate faster? Why?
Draw a restriction map based on the fragments resulting from the following restriction digestions of a 3 kb plasmid
Discuss, in detail, the role played by DNA and RNA in genetic diseases. What are the breakthroughs, if any, in the treatment/management of genetic diseases?
Which of these statements can apply to endocrine disruptors.
Research a genetic disease that may be cured through the use of genetic engineering. What techniques are being explored to correct the problem? How do concerns of designer embryos stem from this technology?
How do glucagon and epinephrine block fatty acid biosynthesis? How does insulin activate fatty acid biosynthesis? How does the chain undergo further lengthening after 16 carbons? Where in the cell does this occur? Specifically describe the enzyme ..
The transformation was successful, however, when my plate with ten uL did not show any colony growth, whereas, my 200 uL plate showed colony growth.
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