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A 2500 base-pair long cDNA of unknown sequence was cloned into the MCS of a plasmid vector that has universal priming sequences adjacent to and on both sides of the MCS. On one side this is called the "forward universal priming sequence" and on the other it is called the "reverse universal priming sequence." Sanger sequencing was done using primers that are specific for each of these universal priming sequences and approximately 700 nucleotides of sequence was determined from each primer. Assuming it is not possible to obtain sequence analyses longer than 700 nucleotides, describe the steps that must be done to obtain the remainder of the sequence of the cloned cDNA.
Hint: There are two very different methods that will accomplish this. One method requires much more effort and is less reliable than the other. Either method will receive full credit.
Describe how structural modification helped the plant adapt to its environment. Assure the plant you select is an Angiosperm. For example, plants like the mosses, pine trees, and ferns are not the Angiosperms.
Which of statements concerning the sperm might be true. These data let you to infer that the species belongs to which protist groups.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
Batch and continuous sterilisation
The prothalli are bisexual, free-living and photosynthetic. At maturity, the prothallus is flat and heart-shaped. Inspect the lower surface for rhizoids, antheridia and archegonia. What is the function of each of these structures.
How do respiration and/or photosynthesis affect homeostasis in a living organism.
The membrane vesicles are next washed, the membranes solubilized and the proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein.
List and describe at least three cellular features of bacteria that could be targeted to inhibit or kill bacterial pathogen. Which is a significant flaw in this experimental set-up.
Suppose that transgenic organisms can be developed to speed nitrogen fixation. How might use of these organisms affect ecosystem.
Discuss these two laws of Thermodynamics and how they relate to the Law of Conservation of Matter. Which of the next may be the path of a protein from the site where its polypeptides are made to its export.
Which of the following illustrates hydrolysis.
Which will you expect to increase significantly from M to G1. What does this tell you about how the cellular information for eye colour is pass on? Describe in detail.
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