Explain the rabbit brain tissue was homogenized

Assignment Help Chemistry
Reference no: EM13498402

Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column. This is an affinity column in which ATP is covalently linked to a polysaccharide bead. This sample is loaded on top of the column, washed with a low-salt buffer, followed by a wash with a high salt buffer. What is the rationale for this procedure and what would the expected elution profile look like? (Keep in mind PFK-1A Mr = 84000 dal, PFK-1B Mr = 80000dal, PFK-1C Mr=86000 dal)

Reference no: EM13498402

Questions Cloud

Explain what rate of disappearance of no2 would be predicted : The decomposition of NO2 by the following reaction at some temperature proceeds at a rate 5.40 x 10-5 mol NO2/L.s when [NO2] = 0.01144 mol/L.
What will his angular speed be when they are tucked in : An ice skater has a moment of inertia of 2.5 kg*m2 when his arms are outstretched and a moment of inertia of 1.0 kg*m2, what will his angular speed be when they are tucked in
Determine the best possible way to account for inflation : Determine the best possible way to account for inflation when considering where to invest your money. Provide specific examples to support your response
Find the charge on the plates before and after immersion : A parallel-plate capacitor in air has a plate separation of 1.50 cm and area of 25.0 cm^2. find the charge on the plates before and after immersion
Explain the rabbit brain tissue was homogenized : Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column.
Compute the position of the image : An object 0.680cm tall is placed 18.5cm to the left of the vertex of a convex spherical mirror having a radius of curvature of 25.0cm. Calculate the position of the image
Evaluate the average speed of car : Imagine that you took a road trip. Based on the information in the table, what was the average speed of your car
Calculate the assessable - deductible amount : Necktie Pty Ltd (Necktie), an Australian resident company, had the following transactions in the year ended 30 June 2012.
Define taxation law : Calculate Caroline's taxable income for the year ended 30 June 2012. Show workings where relevant and briefly explain all inclusions and exclusions.

Reviews

Write a Review

Chemistry Questions & Answers

  Explain the standard enthalpies of combustion for ethanol

The standard enthalpies of combustion for ethanol and dimethyl ether are -1367kJ mol-1 and -1460kJ mol-1, respectively, at 298K. what is delta U at 298K for the purposes below:

  Explain what is the final pressure of the cs2

The volume the vapor occupies is 300 mL and the pressure of the vapor is 400 torr. The piston is then moved so that the vapor occupies 600 mL, and the system is allowed to come to equilibrium at 28 C. What is the final pressure of the CS2 in the v..

  Draw the appropriate titration curve for the tripeptide

Draw the appropriate titration curve for the tripeptide Met-Lys-Val, starting at pH 1 and ending at pH 12, On the curve label the pKas and the pI

  State gas particles move in a zigzag pattern

Which of the following statements related to kinetic-molecular theory of gases is correct for an ideal gas? A. Gas particles move in a zigzag pattern until they collide with something.

  The hydrogen gas generated is collected over water at

2 nas 2 h2ol 2 naohaq h2gthe hydrogen gas generated is collected over water at 25.0degc. the volume of the gas is 535

  Explain the molar absorptivity coefficients for dye a

A sample contains a mixture of two dyes, A and B. The molar absorptivity coefficients for dye A are 4,875 M-1cm-1 at 628nm and 2,518 M^-1cm^-1 at 684 and for dye B are 1,578M^-1cm^-1 at 628nm and 11,543M^-1cm^-1 at 684nm. In an unknown mixture of ..

  Define trans isomers and constitutional isomer

Does (CH3CH2)2C=CHCH2CH3 have any cis/trans isomers and if so what are they? Constitutional isomers? Can you sketch

  Write the difference between geometric arrangement of

1- what is the difference between the geometric arrangement of electron pairs and the geometric arrangement of atoms?

  Compute equilibrium partial pressure

Given total pressure and equilibrium constant, calculate equilibrium partial pressure, The heterogeneous equilibrium process

  The gas-phase dipole moment of the hydroxyl radical

The gas-phase dipole moment of the hydroxyl radical

  Determine how much heat is released

Determine how much heat is released at constant pressure if 471.0 g of NO2 is produced,?

  Calculate the standard reaction enthalpy for the reaction

Calculate the standard reaction enthalpy for the reaction above (as written) using the following data: N2(g) + O2(g) -→ 2 NO(g)

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd