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You have recently been hired by a research team that studies cystic fibrosis (CF), a fatal disorder that clogs the lungs and other organs with a viscous, sticky mucous that interferes with breathing and digestion. The leader of your research team has informed you that a major fraction of the mucous in CF patients consists of filamentous actin. His hypothesis is that this actin may play a key role in interfering with breathing and digestion in CF patients. In order to test your laboratory skills and knowledge he decides to give you a quiz.
Your first assignment is to assemble actin monomers into filaments. In a test tube you mix actin monomers with KCl, MgCl2, and ATP and then measure filament assembly
Your boss immediately notices that there is a lag phase before actin filaments can be detected and that the "%actin in filaments" plateaus at less than 100%.
Explain the lag phase and why the percent of actin in filaments peaks at a value less than 100%
why the average person secretes about 2.5 litres. where C2 is the greater concentration. If the process of pumping H+ ions is 20% efficient, how many moles of ATP are used each day to secrete gastric juice? How many kilograms could be lifted one me..
What organism produces the disease and how. Why is Anthrax such a potent weapon of bioterrorism? What are its characteristics that make it so.
calculate the final dilution factor.
Your assignment, if you want to accept this, is to cross two of the F1 offspring and complete Punnett square for this cross and then write the resulting genotype and phenotypes. This must be 100 percent completely done for any extra credit. Your cros..
Proportion of Australians aged twenty-five years and over with high blood pressure, by age group, 1999-2000. Note: High blood pressure is defined as systolic blood pressure of 140 mmHg or more;
Identify the problem with this statement: PCR-based systems often yield results for a tiny sample of relatively poor-quality DNA?
An experiment was performed to find out the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditi..
During exercise does the sympathetic nerve activity increase or decrease. What does the parasympathetic nerve activity do?
A new blood-typing system was discovered in which two antigens, P and Q, are determined by a different allele of a gene named N. The alleles of these antigens are about equally frequent in the general population. If the Np and Nq alleles are codom..
Ethanol is supplied in a 95 percent solution. You need 50mL of 70 percent Ethanol for your experiment. How would you make this solution?
In the shotgun approach to whole-genome sequencing, random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence.
How is variability, diploid, independent assortment, crossing over, out breeding mechanisms, polymorphism, heterozygote superiority, hybrid vigor, clines and ecotypes, protogenes, and gene amplification and Pleiotropy relate.
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