Explain include the amounts of tris-base and edta

Reference no: EM13515057

Outline the steps necessary to properly prepare 100ml of TE buffer. Include the amounts of Tris-base and EDTA, and all steps necessary to adjust the pH, including when you add what bolumes of water. Tris-base has a molecular weight of 121 g/mol and will be very basic when it dissolves. EDTA's molecular weight is 372.2 g/mol.

TE= 10 mM Tris buffer, pH 8.0, 2mM EDTA

Reference no: EM13515057

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