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Which of the following is the best reason as to why the strands of a DNA molecule are more easily separated at pH > 11?
A) At pH 11 the cross-strand negative charge repulsion becomes so strong that the two strands are much more easily separated.
B) Most biomolecules precipitate when the pH is greater than 11. Upon precipitation the two strands are much more easily separated.
C) Any solution above pH 11 is considered highly caustic and therefore many of the covalent bonds, especially between the nitrogenous bases and the C1' sugars, will be hydrolyzed at this extreme pH.
D) The pKa values for some of the hydrogen bond donor groups (e.g., R-H2) are lower than 11 and therefore these groups will become deprotonated at pH values above 11. The deprotonated form of these groups cannot form the favorable cross-strand hydrogen bonds that hold the two strands together.
Draw line-bond structures for C3H6O, include all the constitutional isomers (except those with carbon-carbon double or triple bonds.) for each constitutional isomers
How many grams of CaCl2 would be dissolved in 1.2 L of a 0.10 M solution of CaCl2?
The gas mixture contains 2.5% sulfur dioxide (SO2) by weight. How many pounds of SO2 are released in one week? (Sig figs matter)
A 245 mL stock solution contains 2.3 M Ca(CN)2. When 100 mL of the stock solution is diluted to 120 mL, how many moles of CN- ions will be contained in the dilute solution?
Why are the crude ester mixtures washed with aqueous NaHCO3?2. You wish to test 9 esters for antitumor activity. One and only one of the 9 esters will actually have antitumor activity. You will prepare the esters from an alcohol and an acid as was..
a star is estimated to have a mass of 2*10´36 kg. Assuming it to be a sphere of average radius 7 * 10´5 km, calculate the average density of the star in units of grams per cubic centimeter.
Describe the health and sfety concerns of using H2S and thioacetamide as the S2- donor. which is used most frequently and why is its use preferential?
calculate the inimum temperature at which this process will be spontaneous under standard conditions. Assume that Delta Ho and Delta So do not vary with temperature.
The mass of a cube of iron is 338 g. Iron has a density of 7.87 g/cm3. What is the mass of a cube of lead that has the same dimensions?
A particular element M has a heat capacity of 0.36 JgK and it forms an oxide that contains 2.9 grams of M per gram of oxygen What is the molar mass of the metal
Calculate the pH of a solution prepared by dissolving 18.32 grams of benzoic acid and 43.23 grams of sodium benzoate in water sufficient to yield 1.00 L of solution.
Calculate the number of moles of solute present in 50.0mg of aqueous solution that is 1.25 molality NaCl.
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