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Question: You are studying the promoter of the rpoA gene in E.coli, which has a -35 consensus sequence of TTGACA. If you mutate the -35 sequence to TTCAGA, will this have any effect on the transcription of the rpoA gene? Why or why not?Question: You create a prokaryotic in vitro transcription system, focusing ot the trp gene in E. coli. To this system, you add all required components for successful transcription in E. coli, plus a compound that adds a third hydrogen bond between all base-paired RNA nucleotides. Will the trp gene be transcribed correctly? Why or why not?Question: If you remove the methyltransferaseenzymes that can add methyl groups to nitrogenous bases, what will happen to mRNA processing in eukaryotes? Why?Question: Construct a eukaryotic in vitro RNA procesing system, focusing on a 23S rRNA in the protist Tetrahymena. To this syste, you add all required components for successful RNA processing in Tetrahymena, except for one of the splieceosome snRNPs. Will the 26S rRNA be processed correctly? Why or why not? Be specific.
Explain the hierarchy of plant structure using the stem as an example organ and follow it through each stage until you reach the level of cell. Only use the vascular tissue systems.
Determine which of the following statements would be inaccurate regarding the discovery of the DNA molecule?
Contained is a description of the signaling cascade that results from EGF stimulation of the Ras-MAPK pathway in cells.
Describe a signaling pathway deregulated in cancer that involves pRb and p53 as players. Have any advancements been made in the understanding of these pathways since the provided review was written?
The pUC series plasmid contains LacZ gene with multiple cloning site. Suppose if you insert a piece of DNA into this LacZ gene, how will you tell which one is your recombinant.
Assume you use phase contrast microscopy to observe a wet mount of a soil sample and a goo sample. In what ways are potential ET microbes similar to microbes previously characterized on earth?
On physical assessment, nurse determines that his ankles and feet are very swollen. Which of these symptoms reflect left-sided heart failure and which reflect right-sided heart failure, and why give appropriate reason.
Which magnification was most helpful in making this determination? What optical principles are involved here and describe the role of each.
They are though more closely related to each other than they are to either of their parents. since now they know their child will not be a hemophiliac! Explain how they know this.
Critically explain the environment and outcome of the environmental problems, the strategies and factors which affect the environment and prevention of the environmental strategies with the graphical explanation.
This problem refers to the MN and ABO loci mentioned in class. It also refers to the Rh locus, which is responsible for the positive or negative part of the blood type.
In Drosophila a cross was made between a yellow-bodied male with vestigial (not fully developed) wings and a wild-type female (brown body). The F1 generation consisted of wild-type males and wild-type females.
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