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Unlocked one of your documents and this is one of the questions i had a question about it. If a double-stranded circular DNA molecule has 4 recognition sites for HindIII, how many fragments will be generated if you digest that molecule with HindIII? How many fragments will result if you linearize the above ds DNA molecule and then digest with HindIII? Explain the answer that you had was--> A ds circular DNA with 4 sites for a restriction enzyme will generate four fragments because one of the sites will linearise it. A linear DNA will give 5 fragments. --> Can you explain it in detail as to how you obtained it? If you can give a step by step how you got the fragments and linear DNA fragments? and the other question i had was this one--If you are tasked with screening a genomic library for a specific DNA sequence and a cDNA expression library for the expression of a specific protein, what kind of screening methods will you use for each and why? the answer you provided for this one was --> A cDNA library can be screened for a particular protein using the protein fragment complementation assay (PCA) technique using Green Fluorescent Protein (GFP) as a reporter. Interactions of a 'bait' protein of interest and 'prey' cDNA fragment brings the fragments of the "reporter" protein in close enough proximity to allow them to form a functional reporter protein whose activity can be measured...How did you get this answer? I have looked all over the book and notes and have yet to get that. Please help. any advice or feedback would be greatly appreciated.
Which of the following statements is true regarding cell-cell adhesion?
List the seven major biomes discussed in the text and give a brief description of the climax vegetation in each.
How would anaphase be disrupted if a key protein in the kinetochore was inactivated?
q1. eastern tent caterpillars malacosoma americanum live in large groups in silk nests or tents which they build in
How would the process of photosynthesis be affected, and in what steps, if light, CO2 concentration, and/or temperature were altered during photosynthesis?
What is EMG (electromyogram) measuring and how might it correlate to muscle force? Describe the physiological reasons that an EMG would sound like a revving engine? and why would prostigmin intensify the sound? and how exactly would postrigmin inf..
Harry is hospitalized with bacterial pneumonia.
A very heavy rainstorm floods a mountain river, changing its course and digging a deep canyon through the soft soils of the meadow in the valley below. What happens to the mice on either side of the valley?
Gel filtration column was calibrated with blue dextran, its elution volume found to be 17.4 mL. Then, unknown protein and BSA were ran through the same column and eluted in 43 mL and thirty-six mL correspondingly.
Make one hundred microliters of an enzyme solution at 0.1 U/microliter. You are given water to dilute the concentrated stock enzyme. The stock enzyme comes at 10 U/microliter.
Citric cycle- Which enzymatic defect in the citric cycle cause low oxygen consumption at succinate substrate, but normal consumption of oxygen at furmarate and malate? Explain.
Which best describes an ecological community? a.intraspecific competition of members of a brook trout population inhabiting a stream during a given year.
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