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lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome. Identified target genes appear to be involved in DNA repair and recombination. Under normal conditions, the LexA protein binds near and represses transcription of target genes; however, when the cell experiences significant DNA damage caused by environmental mutagens, the LexA protein is inactivated and transcription of the target genes occurs. Your research objective is to identify all of the target genes repressed by the LexA protein. (Assume that you have access to a DNA microarray consisting of sequences representing each of the genes in the E. coli genome.) As the starting point for your work, you isolate a lexA mutant strain that produces no functional LexA protein. Briefly describe an experiment that uses a functional genomics approach to identify all of the LexA target genes. Give the basic steps of the experiment and show how it would allow you to achieve your objective.
Blood glucose was monitored weekly for all mice. After five weeks, there was no change in average body weight between groups.
The movement of glucose into a cell against a concentration gradient is most likely to be accomplished by which of the subsequent. movement of glucose into the cell through a glucose channel.
The result of mitosis is that daughter cells end up with similar number of chromosomes just as the parent cell.Explain how the results of mitosis differ between two processes of cell division?
Researchers analyzed rates of twin births in the Sami population of Northern Scandinavia during the 18th and 19th centuries.
explain the advantage and disadvantage of random addition or deletion of nucleotides at VDJ junctions during re-arrangement of immunoglobulin gene segments.
Suppose that the genes required for Isoleucine biosynthesis are contained within an operon. If the cell is grown in the presence of high values of isoleucine you would expect to find,
How can altering ice nucleation protein production yield a bacterial strain useful to agriculture? Explain the logic and how this works.
An organism of the genotype AaBbCc was testcrossed to triply recessive organism. The genotypes of the progeny are presented in the following table.
Determine how to calculate osmolality for an electrolyte versus nonelectrolytes and molality versus osmolality.
Imagine looking during a microscope at a squashed onion root tip. The chromosomes of many of cells are plainly visible. In some cells, replicated chromosomes are aligned along center (equator) of the cell. These particular cells are in which stage of..
Required a list of equipment nessessary to complete it as well as a method to isolate the cell parts, how much protein is in the red blood cell as well as the amount of DNA in the cell.
Explain how adult stem cells can sense and respond to the nutritional status of an animal. Explain what kind of cues an adult stem cell can respond to, and where these cues are coming from.
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