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The following data was obtained in three different sets of experiments utilizing an enzyme obeying the Michaelis-Menten rate law; the first set ofexperiments was performed in the absence of inhibitor, the second and third sets inthe presence of a 10 mM concentrations of two different inhibitors (I1 and I2). Thetotal enzyme concentration was constant throughout.vo (μMs–1)[S] (mM) [I] = 0 [I1] =10 mM [I2]= 10 mM1 2.5 1.17 0.772 4.0 2.10 1.255 6.3 4.00 2.0010 7.6 5.7 2.5020 9.0 7.2 2.86a) Determine KM and Vmax for the enzyme. For each inhibitor,determine the type of inhibition as well as the constants Ki and/or Ki′. Whatadditional piece of information is required to calculate the turnover number of theenzyme?
b) If [S] = 5 mM, calculate the fraction of enzyme molecules thathave a bound substrate i) in the absence of inhibitor, ii) in the presence of 10mM I1, and iii) in the presence of 10 mM I2.
q1. chloramphenicol is the antibiotic that targets prokaryotic 70s ribosomes but not eukaryotic 80s ribosomes. which of
In vascular plants, how is the sucrose-rich phloem saptransported? Water can enter roots through two pathways, apoplastic andsymplastic routes (see Figure 36.12). How do these pathwaysdiffer?
What is the role of DNA gyrase in transcription.
In 1918, the tsar and tsarina of Russia and their five children were presumably assassinated and buried in an unmarked grave. Later, several women came forward and claimed that they were in fact Anastasia.
Two genes, designated A and B, are located 10 mu from each other. A third gene, designated C, is located 15 mu from B and 5 mu from A. A parental generation consisting of AAbbCC and aaBBcc individuals were crossed to each other.
How might 32 P and 35 S be used to demonstrate that the transforming principle is DNA? Briefly outline an experiment that would show that DNA rather than protein is the transforming principle.
750-1050 words in which you discuss generally accepted accounting principls relating to health care.inclue a brief
On the coding strand above, indicate where you would expect FRP to bind to the DNA. In one sentence, describe why the decision makes sense.
How would you interpret a negative Slide Test and a positive Tube test using the same organism? What is the role of sterile saline plus organism on the Slide Test?
What is mechanoreceptor and i wana detail about it that how it function in non chordate with dramatically represent with several examples msc level
q1. why is it that i feel so tired right after eating a big meal except have a bunch of energy about 2 hours after a
Describe the process of genetic recombination in respect to the manner by which a vast diversity of antibody specificities is produced by a relatively small number of genes.
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