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In general, what length of oligonucleotide would be sufficient to amplify a single unique sequence--that is, what would the minimum length have to be in order for the oligonucleotide sequence to be present only once in the genome if every possible sequence were equally represented? To simplify your calculation, assume that all bases occur with an equal frequency. Express your answer as an integer.
The compound ouabain is a specific inhibitor of the active transport of Na+ out of the cell. For each of the following precesses in your body, would you expect the process to be sensitive to inhibition by ouabain?
Explain how to make 50 ml of the following 10X Gel electrophoresis loading buffer, 100 mM EDTA (pH 8.0), 0.1 percent and find mature mRNA.
A microbiologist found a new restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced.
Assume a migrating salmon moves from Alaskan waters past Cabo San Lucas into the Sea of Cortez, many miles south of San Diego.
Explore the role of the epigenome and/or other gene expression regulatory mechanisms in the development of cancer. Discuss in detail one possible epigenetic/post-transcriptional/post-translational target for cancer treatment, and identify the succes..
Two genes located on separate autosomes determine flower color and shape. The gene for flower color exhibits incomplete dominance, where the color can be red.
You repeat Snyder's experiment, but instead of determining the ability of each family member to taste PTC, you determine what alleles they carry: 2 PAV (PAV/PAV), 2 AVI (AVI/AVI),
Using the data given in table below draw a Lineweaver-Burk plot.
In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structure of hydrogen and helium.
The global community is plagued by increasing incidence of lung cancer, colorectal cancer, breast and pancreatic cancers, prostate cancer, leukemia, non-Hodgkin lymphoma, liver, ovarian and esophageal cancers.
Discuss the difference between DNA methylation and histone methylation? I understand that both include the repression of genes and that DNA methylation occurs at CpG islands.
If a cell with 2n=4 chromosomes failed to separate homologous chromosome pairs correctly during meiois, so that one pair went to one daughter cell and other cell received no copy,
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