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I want to design a diagnostic test to distinguish a mutant allele from a wild type allele, as in the two allele depicted below. I have the choice of using two restriction enzymes (A and B) with target sites marked by the placement of A and B on th etwo alleles below. The resultant fragments friom the digestion of the two alleles are indicated below in parantheses. Suppose you design a PCR test for detecting one or the other allele using a single set of primers. For your test, which of the following represent all valid choices of restriction enzymes to detect a difference between teh two alleles?___(100)__A__(300)__A__(100)___ WT_______(500)____B____(500)_____ MutantA. only AB. only BC. neither A nor BD. A and BE, you can only use A and B together
What are the four most important elements necessary for life on this planet? Name the four most important groups of organic molecules for life? Give an example of each.
Given the function of chloroplasts, which of the following best describes a cell in the root of a particular plant?
Dialysis patients, who will have blood withdrawn, dialyzed, then replaced, are always weighed while they enter the facility and then weighed carefully before they leave. What is the most probable explanation for this requirement.
A patient was diagnosed with a UTI caused by E. coli. Explain the immunological course of events during the infection, development of the disease, an recovery of the patient.
If a GM crop was engineered to produce growth hormone, what are some of the possible ethical outcomes and questions that would need to be anticipated before using the re-engineered crop?
what is the ultimate source of plate tectonics? a.the sun b.global magnetic fields c.thermonuclear reactions d.gravity e. house music
In constructing an oligonucleotide probe based on information from a protein's sequence, The synthesis of most proteins starts with methionineor a derivative there of, so a methionine codon assures one will start at the beginning of the gene
gene therapy is a major area of research for curing a variety of genetic diseases. briefly describe the process of gene
What does information suggest about the transmission.
q1. hemoglobin is 50 percent saturated with oxygen when po2 26 torr i.e. p50 26 torr . if hemoglobin exhibited
As an adult your DNA replicates billions of times and undergoes cell division. In the face of extreme challenge, DNA polmerase does a remarkably good job of inserting proper nucleotide at each spot in response to template. Though, mistakes occasio..
Estimate the value of the turnover number of the enzyme invertase, given that Rmax (Vmax) for invertase = 15.8 mmol·L-1·min-1 and [E]t = 4.8 ?
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