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You have purified a protein, MT1, that binds only to GTP-bound tubulin. You proceed to fuse MT1 to GFP and find that the fusion of GFP to MT1 does not disrupt MT1 function in any way (good news!). You use a different dye to label the microtubules in red so that you can visualize MT1-GFP and the microtubules at the same time. (Note that the red dye does not affect the microtubule's properties.)
A. If you were to polymerize some red-labeled microtubules in a dish, would you expect MT1-GFP to be associated with one or both ends of a microtubule, with the middle of the microtubule, or be evenly distributed throughout the microtubule? Explain.
B. If you were to take time-lapse images in which you visualize only MT1- GFP (and not microtubules), what would you expect to see? Describe what you think would happen to MT1-GFP over time with respect to its localization inside of this cell.
The condition "xeroderma pigmentosum" is characterized by high rates of skin cancers like malignant melanoma. It is caused by mutations in any of many genes that encode enzymes that participate in:
what are the similarities and differences of eukaryotic and prokaryotic dna synthesis in chart form.
What is the role of the hepatic portal system?
What changes in the genome would we expect if the size of a organism physically changes? What would be the resulting observation in the world around us if this size change were a genetic alteration?
your hair is composedof protein known as keratins that have an alpha helix- like fibrous structure. the alpha helix-structure has approximately 3.6 amion acids per turn of of the helix and a linear dimension of 0.54nm per turn of the helix.
To ameliorate antibiotic resistance, several individuals have lowered or eliminated the usage of certain antibiotics. However this has not worked most of the time.
A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Sketch a plasmid map.
The final result in the PM + DQA1 test is seen as blue dots on a white strip. Why are the positive and negative controls so important in this test?
Describe how you would sample two different jars in a lab experiment. For example, how would you sample a jar with extra fertilizer versus one with extra organic matter
How can caffeine cause a muscle fiber to contract and generate force, but without generating an end plate potential or action potential of the membrane?
How is glycolytic flux increased during a bicycle exercise test biochemically in someone who is exercise intolerance?
Why are malnourished people often more susceptible to colds and other infectious diseases?
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