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A kan-r, tet-r plasmid is treated with the restriction enzymeBglI, which cleaves the kan (kanamycin) gene. The DNA is annealed and ligated to a BglI digest of Neurospora DNA and thenused to transform E. coli.
a. What anitbiotic would you put in the growth medium to ensure that each colony has the plasmid?
b. What antibiotic-resistance phenotypes will be foundamong the resulting colonies?
c. Which phenotype will contain Neurospora DNA inserts?
How and why does muscle contraction, increase in enzyme activity (LDH) and SNS activation contribute to lactate production during exercise? Cite each of the sources used.
What combination(s) of chromosomes are possible in haploid cells following both divisions of meiosis? (Hint: remember independent assortment). A diploid cell contains three pairs of homologous chromosomes designated C1 and C2, M1 and M2, and S1 and S..
Explain how variables such as social interactions, cognitive processes, environmental variables, cultural context, and biological factors shape what social psychology is all about and how it is practiced.
Discuss how might you expect the muscle fiber and metabolic characteristics to change for an individual training to become a track sprinter
create an outline or flow chart of the categories and subcategories of the steroid hormones. Indicate the glands that secretes each hormones.
Jose cultivates a variety of plants for the fabric industry and is interested in genetically engineering fiber cell types. What cells type would he focus upon?
What are the three central elements of chemiosmotic theory for coupling oxidation to phosphorylation in mitochondria?
Explain why water forms nearly spherical droplets on the surface of a freshly waxed car. Why doesn't water bead on a clean windshield?
In Drosphila, vestigial wings (vg) are recessive to normal long wings (vg+), and the gene for this trait is autosomal. The gene for the recessive white eye trait (w) is on the X chromosome. Suppose a homozygous white, long-winged female fly is cro..
Are the cells observed in your blood smear different that the bacterial cells found on yogurt slides? Why or why not?
We are told that every surface we touch is teeming with bacterial cells, and bacteria are found in the pools we swim in, water we wash with, and on the hands of friends. Why don't we always have problems with bacterial infections all over our bodies.
IF 15% of the nucleotides in a DNA molecule contain guanine, what perecntage of the nucleotides contain each of the other three bases? Explain your reasoning.
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