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1. Severe combined immunodeficiency disease (SCID) is fatal at an early age. SCID is caused by the lack of the enzyme adenosine deaminase, which is encoded by the gene ADA. Suppose you want to use a gene therapy method to treat the disease. You have DNA that contains the ADA gene and a virus that infects human cells. Design a protocol to get the ADA gene into the viral DNA.
2. Define restriction fragment length polymorphism (RFLP) analysis. The information generated from RFLP is useful in criminal investigations. Suppose some DNA samples are taken from a crime scene, suspect, and the victim. The samples are amplified by PCR, and then subjected to RFLP analysis. Below is a gel electrophoresis of the RFLP results. D1 is from defendant 1; D2 is from defendant 2; V is from the victim; and S1, S2, and S3 are from the crime scene. Speculate on how these results would have an impact on each defendant.
3. Define genomics and proteomics. Name two techniques that are used in each field of study. Protein is translated from mRNA, which is transcribed from the gene. However, the proteome is much larger than the genome for a given organism. Give two reasons why there are more proteins than type of genes.
q. after prolonged exposure to isoproterenol that binds to b-adrenergic receptors and activates adenylate cyclase cells
q1. examine how the pancreatic endocrine hormones glucagon and insulin work antagonistically to maintain homeostasis in
Mutational changes were induced in a single codon in the gene of T4 phage. The following amino acid substitutions were observed. By following the base changes in the codons, determine the codon for each mutant.
What is the process of transcriptional initiation?
I am working on a scientific paper for the identification of an unknown bacterium. I would like someone that could look over my paper and give me any tips. I am almost finished and my paper needs to be at least 3 pages long not including charts or..
When a yellow female Labrador retriever was mated with a brown male, half of puppies were brown and half were yellow. The same female, when mated to a brown male produced all brown puppies.
describe the processes and stages of human embryogenesis.What factors will disrupt this normal growth and cause problems?
A population of Anopheles mosquitoes reaches very high densities, and is subjected to aerial spraying by DDT. The following six years, the mosquitoes are not observed at all, but the population was sprayed “just in case”. An outbreak occurs sevem ..
How much percent of water and oxygen is there on planet earth?
Determine the pathophysiology for intrinsic vs extrinsic asthma? Are they both IgE mediated and Write out the overall chemical reaction of cellular respiration.
Briefly describe the methods used by bacteria and fungi to tolerate environmental heavy metals. Include references and your views on the potential commercial applications of bacteria and fungi.
Based on your knowledge of M-Cdk regulation, indicate two ways the activity of this essential complex might be shut down if DNA damage were detected.
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