Cuvette with a solution of enzyme

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Reference no: EM131422204

You fill a cuvette with a solution of enzyme, substrate, and dye that changes color as the reaction occurs. You put it in a spectrophotometer and record the absorbance at several time points. The absorbance increases at first, then starts to level off, staying almost exactly the same at each time point. Why did the absorbance increase at the beginning AND why did it level off over time?

Reference no: EM131422204

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