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To determine the crystal structure of bacterial lactose permease, a mutation that changed cysteine 154 to glycine was engineered. This mutation locked the protein into a particular conformation, so that the transporter was inward-facing such that its sugar-binding domain faced the cytoplasmic portion of the cell. Explain why using this C154G mutant made it more feasible to determine the structure of lactose permease.
a 42-year-old female presents with metabolic acidosis from starvation.1. what might be her symptoms in metabolic
Suppose that you have uncoupled mitochondria (no chemiosmotic gradient to restrict respiration). Which of the following substrates should produce the fastest rate of respiration?
You are given 1 ml of a 50 mM stock solution of NADPH. You are told to dilute it so that its absorbance in the spec 20 equals 0.80. The molar extinction coefficient for NADPH is 6220 liters per mole cm and the path length is 5 cm. The volume of th..
describe using diagrams the component parts of an absorption spectrophotometer and explain the importance of each in
How do the semicircular canals determine the direction of acceleration of the head? Why is there efferent innervation from the central nervous system the outer hair cells?
What changes take place in neurotransmitter release from the rods or cons when they are exposed to light?
Draw a restriction map based on the fragments resulting from the following restriction digestions of a 3 kb plasmid
A scientist sequences both strands ofa DNA molecule and determines that 30% of the nucleotides areadenine. The composition of the other nucleotides mustbe
As a 1st step towards cloning the ding-dong gene from Drosophila, you find a transposon insertion within the gene.
The roles of biological membranes in eukaryotic cells include whichof the functions listed below?
Illustrate the texture and arrangement of the lungs of the cat. If you were asked to create a karyotype from the chromosome spreads you made from HeLa cells, list materials you could use and describe how you would create the karyotype.
A recent headline on the sports page of the newspaper read, "Football player dies awaiting leg surgery." The player was expected to be sidelined for a matter of weeks with a broken leg but died suddenly due to lung hemorrhaging and inability to ox..
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