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1. Complete the following purification table. Which step gives the best purification?
2. Define and/or explain the significance of All of the following.
a. Explain why a fluorescent amino acid, whether naturally occurring (tryptophane) or artificial (Aladan), might be of advantage in Biochemistry.
Step
Total VoIn me
Total Protein (mg)
Total Activity (units)
Specific Activity (units/mg)
Yield (%)
Purification (n-fold)
Crude Cell extract
2,800
70,000
2,700
Ammonium Sulfate
3,000
25,400
2,300
Heat Treatment
16,500
1,930
DEAE Chromatography
80
390
1,630
CM Cellulose Chromatography
50
47
1,350
Bio-Gel A Chromatography
7
35
1,120
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