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Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs. pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 x 105 sec–1 .1. Calculate the fraction of the total enzyme active sites in solution that would be in the active form at pH 7.2.2. Calculate what kcat would be if the activity of the enzyme were measured at pH 7.2.
Write down an equation for the complete oxidation of glucose. What does this imply about the volume changes you would view if you simply sealed the pea seeds in a flask.
Sinorhizobium meliloti can be protected from osmotic stress by the presence of certain disaccharides. Dan Orr found that sucrose does a fine job as an osmoprotectant for our strain of S. meliloti,
mycobacterium avium-intracellulare is prevalent in aids patients. in an effort to determine the source of this
What would be the free energy for proton movement from the intermembrane space to the matrix in a mitochondria under the following sets of conditions (please put answers in kcal/mole, and assume room temperature of 25° C).
Why in the world plants evolved such beautiful colours and shapes and create such wonderful scents. What do you tell her.
Assume that the genes are located on separate chromosomes. An F1 is produces by crossing the original mutant A with the original mutant B. This F1 is testcrossed: What propotion of testcross progeny will have no trichomes?
The autosomal recessive gene transformer (tra) mutation converts chromosomal females into sterile males. A female Drosophila that is heterozygous for the tra allele is mated with a normal male homozygous for transformer. What is the sex ratios of the..
If we add 100 ul of 0.5 mg/ml ConA and 100 ul of 1 M mannose to 800 ul of a dilute suspension of red blood cells in ConA buffer, what should we expect to happen
A husband and wife have normal vision, although both of the couple's fathers are red-green color-blind, an inherited X-linked recessive condition. What is the probability that the couple's first child will be a normal daughter?
The citric acid cycle is described as the major pathway of aerobic catabolism, which is an oxygen- dependent degradative process. However, none of the reactions of the cycle directly involves oxygen as a reactant. Why is the pathway oxygen-depende..
Assume you are researching microbial life in the Rocky Mountains. You scoop up a water sample from a melted snow puddle because you are curious about the possible microbes that might live there.
The two biochemical pathways for coat color pigment in a species of triblles are shown below. The final color of the tribble is the combination of colors produced by the two pathways.
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