Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
You want to make an antibody against a C. elegans nematode protein that the DNA shown in Figure A encodes. To do this, you will clone the coding sequence into a bacterial expression plasmid, overexpress the protein, purify it, and inject it into a mouse to stimulate the production of antibodies against the protein.
A. You use polymerase chain reaction (PCR) amplification to amplify the DNA corresponding to the 1800 base pairs of coding sequence; this coding sequence lies between the two flanking sequence shown in Figure A. You will then insert the PCR product into the BamHI site of the expression plasmid shown in Figure B. What are the sequences of the two oligonucleotide primers that will allow you to amplify the DNA by PCR and insert it into the BamHI site? (Remember to indicate the 5' and 3' ends of the primers.)
B. Briefly describe the series of enzymatic treatments you will use to create the expression plasmid before transferring it into bacterial cells.
C. Several weeks after you inject a mouse with your purified protein, you sample its blood serum, which contains antibodies. You know that the mRNA corresponding to your protein is found in gonad cells but not in gut cells. To test whether the mouse has made antibodies against your protein, you isolate protein from gonad cells and from gut cells and perform a Western blot (also known as an immunoblot) with the mouse serum and a fluorescent second antibody that binds mouse antibodies. Your result is shown in Figure Did the mouse make antibodies against your protein? Does the serum specifically recognize only one nematode protein? (Illustrate your written answers by labeling the blot.)
Create a an experimental setup that would test hypothesis that people who carry rabbit's feet have good luck in term of avoiding colds and the flu
what would be the partial diploid genotype for a strain that would produce B-galactosidase constitutively and permease inducibly?
In what kind of micro environments would we find bacteria and archaea?
How does the structure of DNA (double helix) determine how the genetic information is passed on? How does the cell use the information contained in the DNA to construct proteins (transcription and translation)?
Draw the response of the post-synaptic cell to three stimuli of different intensity (gentle, medium, and intense). Be sure to label all axes.
Two dissimilar inbred strains of mice, A and B, are crossed to yield an F1 hybrid strain, AB. Suppose if a large dose of spleen cells from an adult A mouse is injected into an adult AB mouse,
q. a student designs an experiment to create an alcohol known as butanol. she mixes a species of clostridium bacteria
What are ten - twenty possible variables that may affect seed germination? Make a table for your data, including title, units, and any other useful information.
suppose you could selectively prevent production of amylase or oligo-1,6-glucosidase in an organism that normally hydrolyzes starch. Which enzyme would the organism miss the most?
how such a mutation might alter the expression of a gene.
When gold beads were injected into a cell to see whether or not they could get into the nucleus itwas discovered that beads bigger than 9 nm in diameter (orbigger than the size of a proteinwith a molecular weight of ~40,000) could not get intothe ..
If an organism has a diploid number of 16, how many chromatids are visible at the end of mitotic prophase?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd