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Assume that a 4000-bp long circular DNA plasmid has two HindIII sites at coordinate 1 and 801. If we digest this circular DNA plasmid with BamHI, we obtain 500 bp, 1400 bp, and 2100 bp DNA fragments. If we perform HindIII/BamHI double digestion with this circular plasmid, we obtain 200 bp, 500 bp, 600 bp, 1200 bp, and 1500 bp DNA fragments. From above information, please interpret the location of BamHI sites.
(1) How many BamHI sites are there in this circular plasmid?
(2) Where are those BamHI sites? Please draw the map.
Can someone help me describe the three different patterns of cell cycling activities and give an example of each? Not quite sure what it's asking for.
Explain why cutting an arterial vessel will cause a greater loss of blood over the same amount of time than cutting a venous vessel.
How many sectors are reported for any partitions on physical device? How many sectors are on whole physical hard drive?
Assume you start with 10 ml of 2% acetic acid, and you add 90 ml of water. We will call the resulting solution "dilution#1". What is its concentration?
Enzymes are typically required to catalyze reactions that would be very unlikely to occur on their own. Suppose that the activation energy, Ea, for the uncatalyzed cheese reaction at 20C is 18kcal/mol.
1. (a)Suppose we wish to determine the structure of a 56-residue protein. Would NMR or X-ray crystallography be likely to be more successful and informative? Why??(b) Same as above, but this time it's a 75 kDa protein.
Suppose that every non sterilized individual makes exactly ten gametes. Determine the total number of gametes?
what happens to the pressure inside a cell that is placed in a hypertonic solution? Does osmosis occur if a cell is placed in an isotonic solution?
Where do the simplest plants and animals live.
What are some foods whose production depends upon lactic acid oralcoholic fermentation? determine which organisms areresponsible, which pathway they are using, and (specifically) what product of fermentation is responsible for the effect on the fo..
What are bypass polymerases? How do they differ from the replicative polymerases? How do their special features facilitate their role in DNA repair?
Ralph is taking scuba diving classes, and is warned not to hold his breath as ascending from depth but to continuously release air from his mouth. What is the basis of this instruction and what is risk if it is ignored.
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