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1. You have just read a paper that the addition of caffeine to cells disrupts the DNA damage checkpoint and you would like to understand why. By searching for proteins that bind to caffeine, you isolate a protein, Caf1. You believe that Caf1 is involved in the DNA damage checkpoint because when you delete Caf1 from yeast cells and add a drug that causes DNA damage, the cells fail to arrest in the cell cycle.You give your Caf1-deleted cells to an undergraduate in the laboratory and ask him to take care of the strain for you while you take the weekend off to celebrate your finding. Upon your return, you find the poor undergraduate in tears. He explains that he thinks that he messed up the Caf1 mutant strain while you were gone because after growing Caf1 and wild-type cells in rich media and examining them during mitosis, Caf1 mutants looked the same as wild-type cells. Are you concerned about the undergraduate student's findings? Why?
2. You are interested in epithelial tissue formation and work in a lab studying Madin-Darby canine kidney (MDCK) cells. MDCK cells spontaneously form hollow epithelial vesicles. Staining these vesicles with an anti-actin antibody demonstrates the existence of apical microvilli near the central cavity of the vesicle. A new postdoctoral fellow in the lab comes to you in a panic. You had given her some dissociated MDCK cells that have been cultured as single cells in a collagen matrix. Because the cells did not look obviously polarized to her, she stained the dissociated MDCK cells to visualize the microvilli and she does not see any signs of polarized microvilli localization in these cells. Explain why you are confident that you did not give her the wrong cell line, and suggest a better experiment that will allow her to test whether the cells you gave her are MDCK cells.
Is a catalase test perfromed on colonies from a BAP? State reasons to support your answer.
Frank found himself in the midst of an ethical dilemma at work, and was using an ethical decision-making matrix, to address his work and personal values. Frank was most likely in which phase of the Ethical Decision-Making Framework.
describe the role of cortisol in the stress responce mite it be usefull in reducing the stress responce prior to surgery?
Discuss how much sample what sized aliquot is required to prepare 500 mL of a 1 to 100 dilution, and how much diluent would you need?
Consider this redox reaction that is involved in fermentation. Answer the following questions based on the forward direction of the reaction as shown below.
At start of abduction of arm, deltoid muscle is assisted by: subclavius., supraspinatus, coracobrachialis, Biceps brachii.
define What would the genotypes and phenotypes. Follow an X-linked recessive mutation from a cross between a heterozygous female and a wild-type male.
He determined that the rising phase of the action potential in ltxnsp neurons was due to a sudden increase in PK. What should be the sign and approximate amplitude of the voltage change of these action potentials? Why?
What happens if an atom of an element gains or loses electrons? What happens if an atom of an element gains or loses neutrons? What happens if an atom of an element gains or loses protons? Please provide a detailed answer.
A steel wire in a piano has a length of 0.5000 m and a mass of 5.200 10-3 kg. To what tension must this wire be stretched so that the fundamental vibration corresponds to middle C (fC = 261.6 Hz on the chromatic musical scale)?
Two strains of the same species both contain known PCR primer sites for a gene that encodes for a virulence factor. However, the avirulent strain has a long insertion mutation in the PCR primer site that is not present within the virulent stain. H..
Briefly explain why digester sludge accounts to 5-10 percent of the total flow but contains 25-30 percent of the organic nitrogen.
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