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Q. Suppose a mammalian genome (4x10^9bp in size) is digested with Hind III, and you are cloning a unique fragment of 2kb.
A. Approximate how numerous clones you will need to screen?
B. If you first size select the genomic DNA, realizing a 10-fold purification, and clone that DNA in a plasmid, now how many clones will you need to screen?
C. If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen?
D. If you screen 5,000 colonies on a 15cm dish, how various dishes will you require to find a clone of interest? "
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Write a scientific review of membrane proteins
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Hermann Kolbe's synthesis of an organic compound, acetic acid, from inorganic substances that had been prepared straight from pure elements was an important milestone for what reason.What piece of evidence contradicts this claim.
Understand the section on spermatogenesis. Do you think the age of the man is as important in determining the chance of chromosomal abnormalities? Why or why not give reasons.
What would be the expected frequencies of the homozygous dominant, heterozygous, and homozygous recessive after 100 generations.
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