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Question: Say you injected and x amount of protein onto the SEC column (size exclusion chromatography) and it produced certain peaks. Now say you repeated the experiment, using the same amount of protein (same concentration as the first) but the second time around the heights of the peaks differ. Can anyone explain to me why would the peak heights differ, even though the same amount of protein was used. Is it due to the fact that some of the protein remained bound to the column during the second run? Here's the question asked by my instructor . . .
Explain why if we injected the same amount of protein onto the SECcolumn, that the heights of the peaks are different - if they are all the same concentration shouldn't they all be the same? Exlpain.
What is a main property of DNase that makes it useful for assessing whether chromatin is in a closed (tightly condensed) or open (loosely packed) configuration.
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the owner of a company that sells cleaning products. The people in research and development have two new products for you to review.
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After a long scuba diving session on a Caribbean reef, Carl boards a plane to Dallas. He begins to feel pain in his elbow on the flight back to Dallas. What is happen to him and why? How does he require to be treated.
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They isolated bacterium in a pure culture and demonstrated that experimental healthy animals injected with this culture became sick. What other test do researchers require to perform to be absolutely sure that the bacterium is responsible for the dis..
Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and next examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What ca..
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