A plate using a technique that produces isolated colonies

Assignment Help Biology
Reference no: EM137000

Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is additional to a mixture containing EcoRI and bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.

Bacteria contain a plasmid into which eukaryotic gene has integrated will grow in what?

Reference no: EM137000

Questions Cloud

Difference between macroeconomics and microeconomics : Explain the difference between Macroeconomics and Microeconomics. Also explain how economics is used as a social science and as a policy tool.
How is the human motor system organized : Describe the structure of atp and gtp. Describe how atp is produced during chemiosmosis. Illustrate two specific cellular processes that require atp and explain how atp is used in each process.
Inability of opec : Producing nations outside the organization, like Britain and Norway, should do their share and cut production.
Variation affect corporations and people : Illustrate now have to lend out how much does this bank if it decides to hold only required reserves.
A plate using a technique that produces isolated colonies : The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin..
Combination of monetary and fiscal policy : If policymakers want to reach full employment while maintaining balanced trade, what combination of monetary and fiscal policy should they use.
Suppose a uniform nucleotide distribution in the genome : What is the relationship between an increase in light intensity and photosynthetic rate in leaves from a corn plant? How does this association compare with what you observed for tomato plants.
Sales manager of an appliance store : Nevertheless your total unit sales have increased over this period. Assuming rational buyers and no deceptive advertising, how can you account for this.
How politics played a role in the discovery of dna structure : Some times in the movie, politics of science were discussed. Explain how politics played a role in the discovery of DNA structure. Do you think the science of today is more or less political than it was during the 1950s? Examine one instance of moder..

Reviews

Write a Review

 

Biology Questions & Answers

  Population of wolves migrates north in search of food

About how long at the earliest is it likely to take before he gets feeling back in that part of thumb. Give two reasons that the wolf population is not at Hardy-Weinberg equilibrium.

  Produce the pigment determining fur color

What color do you guess that the fur of these mice will be? Why with reason?

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  What is the genus of bacteria

You are conducting field work and discover two groups of squirrels living on opposite sides of a lake. How would you plan a study to find out whether these two groups belong to the same species.

  At what time the planet''s population reaches 10,000

If one assumes that Hardy-Weinberg equilibrium applies to population of colonists on this planet, about how many people will have attached ear lobes at what time the planet's population reaches 10,000.

  How denaturing a protein alters the functions of protein

How denaturing a protein alters the functions of that protein. If we are bleeding by the accident, why the injury has to be deeper than epidermis.

  What is the oxygen-hemoglobin saturation curve

Ralph is taking scuba diving classes, and is warned not to hold his breath as ascending from depth but to continuously release air from his mouth. What is the basis of this instruction and what is risk if it is ignored.

  Explain the main types of extracellular signaling

Explain the main types of extracellular signaling.

  Mcdonalds granted to copeland the franchise in omaha

Schupack attempted to exercise right in first refusal McDonalds would not recognize the right in Schupack, claiming that it was personal to Copeland and so non-assignable without their consent. Schupack brought an action for specific performance, req..

  Describe how vesicular transport occurs in cells

What would happen if one were to inject both the R strain and heat-killed S strains into a host organism such as the mouse.

  What will be the change in free energy

Women's bladders, on average, are smaller than those of men. The distribution of bladder volume in women is approximately N (400, 75) in milliliters. Find the proportion of women's bladders with a volume between 500 and 600 ml.

  Utilize the results from replica-plating to reply

The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Utilize the results from replica-plating to reply the questions.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd