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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is additional to a mixture containing EcoRI and bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria contain a plasmid into which eukaryotic gene has integrated will grow in what?
About how long at the earliest is it likely to take before he gets feeling back in that part of thumb. Give two reasons that the wolf population is not at Hardy-Weinberg equilibrium.
What color do you guess that the fur of these mice will be? Why with reason?
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
You are conducting field work and discover two groups of squirrels living on opposite sides of a lake. How would you plan a study to find out whether these two groups belong to the same species.
If one assumes that Hardy-Weinberg equilibrium applies to population of colonists on this planet, about how many people will have attached ear lobes at what time the planet's population reaches 10,000.
How denaturing a protein alters the functions of that protein. If we are bleeding by the accident, why the injury has to be deeper than epidermis.
Ralph is taking scuba diving classes, and is warned not to hold his breath as ascending from depth but to continuously release air from his mouth. What is the basis of this instruction and what is risk if it is ignored.
Explain the main types of extracellular signaling.
Schupack attempted to exercise right in first refusal McDonalds would not recognize the right in Schupack, claiming that it was personal to Copeland and so non-assignable without their consent. Schupack brought an action for specific performance, req..
What would happen if one were to inject both the R strain and heat-killed S strains into a host organism such as the mouse.
Women's bladders, on average, are smaller than those of men. The distribution of bladder volume in women is approximately N (400, 75) in milliliters. Find the proportion of women's bladders with a volume between 500 and 600 ml.
The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Utilize the results from replica-plating to reply the questions.
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